| CatalogCode: | NB110-57567 |
| ProductName: | SHC1 Antibody |
| Product Description: | Rabbit Monoclonal anti-SHC1 (EP332Y) |
| Clone: | EP332Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues in the SH2 domain of human SHC1 was used as immunogen. The antibody detects p46, p52 and p56 |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2,000Flow Cytometry: 1:80IP: 1:50 |
| Background: | The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type (1-3). Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span (4-5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IP, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Pelicci et al. Cell 70: 93-104, 1992. 2. Ventura, A., Luzi, L., Pacini, S., Baldari, C. T., and Pelicci, P. G. (2002) J. Biol. Chem. 277, 22370-22376 3. Migliaccio et al. (1997) EMBO J. 16, 706-716 4. Migliaccio et al. Nature 402: 309-313, 1999 5. Aronheim A, Engelberg D Li N, Al-Alawi N, Schlessinger J and Karin M (1994), Cell, 78, 949-961. |
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