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| Catalog Code: | NB110-57566 |
| Product Name: | SGK Antibody |
| Product Description: | Rabbit Monoclonal anti-SGK (Y238) |
| Clone: | Y238 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues of human SGK was used as an immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1: 500IHC: 1: 50Flow Cytometry: 1:30IP: 1: 50 |
| Background: | Serum and glucocorticoid-regulated kinase (SGK-1) is a 50kDa Serine/Threonine kinase that plays a role in the regulation of epithelial Na+ transport. Two additional SGK isoforms have been identified (SGK2 and SGK3). SGK is regulated by phosphorylation at Thr256 and Ser422 by the phosphatidyl-dependent protein kinase -1 (PDK1) and PDK-2 respectively (1-2). Nedd4-2 regulates SGK activity by catalyzing its ubiquination and degradation. SGK also regulates the binding of Nedd4-2 to the epithelial Na+ channel (ENaC) protein by phosphorylating Nedd4-2 which helps increase Na+ transport (4). While environmental stresses increases its expression, SGK is known to play a role in promoting cell survival, proliferation and differentiation (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.5% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Serine/threonine protein kinase SGK antibody, anti-Serine/threonine protein kinase Sgk1 antibody, anti-Serum and glucocorticoid regulated kinase antibody, anti-Serum/glucocorticoid regulated kinase 1 antibody, anti-Serum/glucocorticoid regulated kinase antibody, anti-SGK 1 antibody, anti-SGK antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Kobayashi, T, Deak M, Morrice N, and Cohen P. Biochem J 344: 189-197, 1999 2. Liu, D, Yang X, and Songyang Z.Curr Biol 10: 1233-1236, 2000 3. Ruifeng Zhou and Peter M. Snyder J. Biol. Chem., Vol. 280, Issue 6, 4518-4523, February 11, 2005 4. Debonneville et al (2001) EMBO J. 20, 7052-7059 5. Leong, M. L., Maiyar, A. C., Kim, B., O'Keeffe, B. A., and Firestone, G. L. (2003) J. Biol. Chem. 278, 5871-5882 |
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