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| Catalog Code: | NB110-57474 |
| Product Name: | Rsk3 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-Rsk3 (pT353/356) (E202) [Thr353/Thr356] |
| Clone: | E202 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Thr353 and Thr356 of human RSK3 was used as immunogen. The antibody only detects RSK phosphorylated on Threonine 353 and 356. |
| Cross Reactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:20,000IHC: 1:50-1:100ICC: 1:50IP: 1:100 |
| Background: | Rsk-3 is a member of a family of 90-kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) that are activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). Rsk-3 is a distinct isoform of p90Rsk that translocates to the cell nucleus, phosphorylates potential nuclear targets, and may have a unique upstream activator (3). Several sites, such as Ser380, Thr359, Ser363 and Thr573, are important for its activation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-90 kDa ribosomal protein S6 kinase 2 antibody, anti-U 2 antibody, anti-MAP kinase activated protein kinase 1c antibody, anti-MAPKAPK1C antibody, anti-p90RSK 2 antibody, anti-pp90RSK 3 antibody, anti-Ribosomal S6 kinase 3 antibody, anti-RSK 3 antibody, anti-S6K alpha 2 antibody, anti-S6K alpha antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Thr353/Thr356 |
| General References: | 1. Dalby, K.N., et al. Identification of Regulatory Phosphorylation Sites in Mitogen-activated Protein Kinase (MAPK)-activated Protein Kinase-1a/p90rsk That Are Inducible by MAPK. J. Biol. Chem. 273: 1496-1505 (1998). 2. Frodin, M. and S. Gammeltoft. Role and regulation of 90 kDa ribosomal S6 kinase (RSK) in signal transduction. Mol. Cell. Endocrinol. 151: 65-77 (1999). 3. Zhao, Y., et al. RSK3 encodes a novel pp90rsk isoform with a unique N-terminal sequence: growth factor-stimulated kinase function and nuclear translocation. Mol Cell Biol. 15: 4353-63 (1995). |
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