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| Catalog Code: | NB110-57470 |
| Product Name: | Rsk-1 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho Rsk-1 (pS380) (E239) [Ser380] |
| Clone: | E239 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human p90RSK1 was used as immunogen. The antibody only detects RSK1 phosphorylated on Serine 380. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:10,000ICC: 1:250-500IP: 1:80 |
| Background: | Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-90 kDa ribosomal protein S6 kinase 1 antibody, anti-dJ590P13.1 antibody, anti-HU 1 antibody, anti-HU1 antibody, anti-MAP kinase activated protein kinase 1a antibody, anti-MAPKAPK1A antibody, anti-p90 RSK1 antibody, anti-p90S6K antibody, anti-pp90RSK1 antibody, anti-Ribosomal protein S6 kinase 90kD 1 antibody, anti-Ribosomal protein S6 kinase 90kD polypeptide 1 antibody, anti-Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody, anti-Ribosomal protein S6 kinase alpha 1 antibody, anti-Ribosomal S6 kinase 1 antibody, anti-Rps6ka1 antibody, anti-RSK 1 antibody, anti-RSK 1 p90 antibody, anti-RSK antibody, anti-RSK1 antibody, anti-S6K alpha 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser380 |
| General References: | 1. Dalby, K.N. et al. Identification of Regulatory Phosphorylation Sites in Mitogen-activated Protein Kinase (MAPK)-activated Protein Kinase-1a/p90rsk That Are Inducible by MAPK. J. Biol. Chem. 273, 1496-1505 (1998). 2. Frodin, M. and Gammeltoft, S. Role and regulation of 90 kDa ribosomal S6 kinase (RSK) in signal transduction. Mol. Cell. Endocrinol. 151, 65-77 (1999). 3. Lazar, D. F. et al. Mitogen-activated Protein Kinase Kinase Inhibition Does Not Block the Stimulation of Glucose Utilization by Insulin. J. Biol. Chem. 270, 20801-20807 (1995). 4. Chen, R. et al. Phosphorylation of the c-Fos Transrepression Domain by Mitogen-Activated Protein Kinase and 90-kDa Ribosomal S6 Kinase. Proc. Natl. Acad. Sci. USA 90, 10952-10956 (1993). |
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