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| Catalog Code: | NB110-57463 |
| Product Name: | Rho Antibody |
| Product Description: | Rabbit Monoclonal anti-Rho (EP487Y) |
| Clone: | EP487Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues near the N-term of human Rho was used as immunogen. The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2,000-10,000IHC: 1:100-250ICC: 1:250-500IP: 1:50 |
| Background: | Rho belongs to the Ras super family of low molecular weight GTPases. Fifteen Rho proteins have already been characterized and divided into three sub-families; the first includes Rho (A, B, C), the second, Rnd 1-3 and the third which includes Rac 1-3, RhoG, Cdc42Hs, Rho/TTF. TC10 and Chp (1-2). Rho acts as a molecular switch which turns on of off various intracellular signaling pathways such as ACK, PAKs, MEKKs ROCK (3). Rho is active when bound to GTP and inactive when bound to GDP (4). It is also known to participate in many physiological activities including cell migration, adhesion, cytokinesis, proliferation, differentiation and apoptosis and to a greater extend cell transformation (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Aplysia ras related homolog 12 antibody, anti-ARH12 antibody, anti-ARHA antibody, anti-H12 antibody, anti-Oncogene RHO H12 antibody, anti-ras homolog gene family antibody, anti-ras homolog gene family member A antibody, anti-RHO12 antibody, anti-rhoa antibody, anti-rhob antibody, anti-rhoc antibody, anti-rhod antibody, anti-RHOH12 antibody, anti-Small GTP binding protein RhoA antibody, anti-Transforming protein RhoA antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Murphy et al. Nature. 384:427-432 2. Yeramian P, Chardin P, Madaule P, Tavitian A.Nucleic Acids Res. 1987 Feb 25;15(4):1869. 3. Maekawa et al. Science 285: 895-898, 1999. 4. Boguski, M.S., and F. McCormick. 1993. Nature. 366:643-654 5. Khosravi-Far, R., P.A. Solski, G.J. Clark, M.S. Kinch, and C.J. Der. 1995. Mol. Cell. Biol. 15:6443-6453. |
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