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| Catalog Code: | NB110-57449 |
| Product Name: | RAF1 Antibody |
| Product Description: | Rabbit Monoclonal anti-RAF1 (Y198) |
| Clone: | Y198 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues surrounding Serine 338-339 of human Raf was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500IHC: 1:50-100ICC: 1:50IP: 1:100 |
| Background: | Raf-1 (c-Raf) is a protein kinase that is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Raf-1 is recruited by and binds to GTP-bound Ras, activating the MEK-MAP kinase pathway (1). Raf-1 is activated via rapid hyperphosphorylation at multiple sites in mammalian cells, including Ser43, Ser259, and Ser621 (2), as well as Ser338, Tyr341, Thr491, and Ser494 (3). p21-activated protein kinase (PAK) phosphorylates Raf-1 at Ser338 in response to Ras, Src and epidermal growth factor (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-cRaf antibody, anti-Craf 1 transforming gene antibody, anti-cRaf antibody, anti-Craf1 transforming gene antibody, anti-EC 2.7.11.1 antibody, anti-Murine sarcoma 3611 oncogene 1 antibody, anti-Raf 1 antibody, anti-RAF antibody, anti-Raf proto oncogene serine/threonine protein kinase antibody, anti-v raf 1 murine leukemia viral oncogene homolog 1 antibody, anti-vraf1 murine leukemia viral oncogene homolog 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Avruch, J., et al. Raf meets Ras: completing the framework of a signal transduction pathway. Trends Biochem. Sci. 19: 279-283 (1994). 2. Morrison, D.K., et al. Identification of the major phosphorylation sites of the Raf-1 kinase. J Biol Chem. 268: 17309-16 (1993). 3. Chong, H., et al. Positive and negative regulation of Raf kinase activity and function by phosphorylation. EMBO J. 20: 3716-3727 (2001). 4. King, A.J., et al. The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338. Nature 396: 180-3 (1998). |
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