| CatalogCode: | NB110-57447 |
| ProductName: | B-Raf Antibody |
| Product Description: | Rabbit Monoclonal anti-B-Raf (EP152Y) |
| Clone: | EP152Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues in human B-Raf was used as immunogen. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000-5,000IHC: 1:250-500Flow Cytometry: 1:50IP: 1:50 |
| Background: | B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | # 94 kDa B raf protein antibody # B raf 1 antibody # B Raf proto oncogene serine threonine protein kinase antibody # BRAF 1 antibody # BRaf antibody # BRAF1 antibody # cRmil antibody # MGC126806 antibody # MGC138284 antibody # Murine sarcoma viral (v-raf) oncogene homolog B1 antibody # Murine sarcoma viral v raf oncogene homolog B1 antibody # p94 antibody # RAFB 1 antibody # RAFB1 antibody # v raf murine sarcoma viral oncogene homolog B1 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Hagemann C., Rapp U. R. Cell Res., 253: 34-46, 1999 2. Heidecker et al 1990 Mol. Cell. Biol. 10:2503-2512. 3. Jaiswal, R. K., Moodie, S. A., Wolfman, A., and Landreth, G. E. (1994) Mol. Cell. Biol. 14, 6944-6953 4. Brose et al. Cancer Res., 62: 6997-7000, 2002 |
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