| CatalogCode: | | NB110-57442 |
| ProductName: | | PTP1B Antibody |
| Product Description: | | Rabbit Monoclonal anti-PTP1B (EP1837Y) |
| Clone: | | EP1837Y |
| Clonality: | | Monoclonal |
| Immunogen: | | Synthetic peptide corresponding to residues near the N-terminus of human PTP1B. |
| Specificity: | | A synthetic peptide corresponding to residues near the N-terminus of human PTP1B was used as an immunogen. |
| CrossReactivity: | | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | | 0.1 ml Rabbit ascites. |
| Uses: | | WB: 1:100,000 - 200,000IHC: 1:100 - 250ICC: 1:100 - 250FC: 1:15IP: 1:80 |
| Background: | | The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3). |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Ascites |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | | 325 |
| AppSummary: | | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-Non receptor tyrosine phosphatase 1 antibody, anti-Protein tyrosine phosphatase 1B antibody, anti-Protein tyrosine phosphatase non receptor type 1 antibody, anti-Protein tyrosine phosphatase placental antibody, anti-PTP 1B antibody, anti-PTPN 1 antibody, anti-PTPN1 antibody, anti-Tyrosine protein phosphatase non receptor type 1 antibody |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Flint AJ, et al. EMBO J 12(5):1937-46, 1993 2. Frangioni JV, et al. Cell 68(3):545-60, 1992 3. Ravichandran LV, et al Mol Endocrinol 15(10):1768- 80, 2001 |
| more information: | | company product webpage |
|