| request information: |
|
| Catalog Code: | NB110-57432 |
| Product Name: | PP2A Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-PP2A (Y307) (E155) [Tyr307] |
| Clone: | E155 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Tyr307 of human PP2A was used as immunogen. The antibody only detects PP2A phosphorylated on Tyrosine 307. Predicted to cross-react with bovine and pig, based on sequence homology. |
| Cross Reactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:5,000IHC: 1:50ICC: 1:50IP: 1:100 |
| Background: | Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-Medium tumour antigen associated 61kDa protein antibody, anti-MGC786 antibody, anti-PP2A alpha antibody, anti-PP2A PR65 alpha isoform antibody, anti-PP2Aalpha antibody, anti-PP2Ac antibody, anti-PP2CA antibody, anti-PPP2CA antibody, anti-Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody, anti Protein phosphatase 2 catalytic subunit alpha isoform antibody, anti-Protein phosphatase 2 regulatory subunit A alpha isoform antibody, anti-Replication protein C antibody, anti-RP C antibody, anti-RPC antibody, anti-Serine threonine protein phosphatase 65 kDa antibody, anti-Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Tyr307 |
| General References: | 1. Janssens, V. and J. Goris. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353: 417-439 (2001). 2. Zabrocki, P., et al. Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol. Microbiol. 43: 835-842 (2002). 3. Milward, T.A., et al. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24: 186-191 (1999). 4. Chen, J., et al. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257: 1261-1264 (1992). |
order or
more information: | company product webpage |
|