| request information: |
|
| Catalog Code: | NB110-57427 |
| Product Name: | POU domain protein 3a (Brn-3a) Antibody |
| Product Description: | Rabbit Monoclonal anti-POU domain protein 3a (Brn-3a) (EP1972Y) |
| Clone: | EP1972Y |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human POU domain protein 3a was used as an immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1000 - 5000IHC: 1:100 - 250ICC: 1:100 - 250FC: 1:90IP: 1:50 |
| Background: | Brn-3a is a transcription factor belonging to the class IV of POU domain transcription factors. It is expressed throughout the peripheral nervous system but especially in postmitotic sensory neurons of dorsal root ganglia. Brn-3a is known to regulate different genes involved in neuronal differentiation and survival (1). Characterization of Brn-3a suggests potential roles of Brn-3a in the development of retinal ganglion cells and sensory neurons, as well as potential roles in the pituitary gland and the immune system. Brn-3a is expressed in the pituitary gland and in a corticotroph cell line (2). The differentiation of the ND7 neuronal cell line to a nondividing phenotype bearing numerous neurite processes is accompanied by a dramatic increase in the levels of the activating POU family transcription factor Brn-3a and a corresponding fall in the levels of the closely related inhibitory factor Brn-3b. Overexpression of Brn-3a in these cells can induce neurite outgrowth and the activation of genes encoding synaptic vesicle proteins in the absence of a differentiation-inducing stimulus (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Calissano M, et al. Oncogene 22(35):5408-14, 2003 2. Gerrero MR, et al. Proc Natl Acad Sci 15(90):10841-5, 1993 3. Smith MD, et al. J Biol Chem 272(2):1382-8, 1997 |
order or
more information: | company product webpage |