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| Catalog Code: | NB110-57426 |
| Product Name: | Plectin Antibody |
| Product Description: | Rabbit Monoclonal anti-Plectin (E398P) |
| Clone: | E398P |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | A recombinant fragment comprising the C-terminal 220 amino acids of plectin |
| Specificity: | A recombinant fragment comprising the C-terminal 220 amino acids of plectin was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Background: | Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry 1:250, immunoprecipitation 1:40, Western Blot 1:5,000, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-EBS1 antibody, anti-PCN antibody, anti-PLEC1 antibody, anti-plectin 1 intermediate filament binding protein 500kDa antibody, anti-PLTN antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Liu, C.G., et al. Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24). Proc Natl Acad Sci U S A. 93: 4278-83 (1996). 2. Wiche, G. Role of plectin in cytoskeleton organization and dynamics. J. Cell. Sci. 111: 2477-2486 (1998). 3. Ruhrberg, C. and F.M. Watt. The plakin family: versatile organizers of cytoskeletal architecture. Curr. Opin. Genet. Dev. 7: 392-397 (1997). 4. Foisner, R., et al. Protein kinase A and protein kinase C-regulated interaction of plectin with lamin B and vimentin. Proc. Natl. Acad. Sci. USA 88: 3812-3816 (1991). |
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