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| Catalog Code: | NB110-57360 |
| Product Name: | PKC mu/PKD Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho PKC mu/PKD (pS744/748) (EP1493Y) [Ser744/Ser748] |
| Clone: | EP1493Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A phosphor-specific peptide corresponding to residues surround S744 and 748 were used as an immunogen. This antibody detects PKC mu phosphorylated on Serine 744 and 748. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1000 |
| Background: | PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. Mutation of serines 744/748 to alanines in the activation loop of intact PKD inhibits its localization to the TGN. Moreover, anti-phospho-PKD antibody, which recognizes only the activated form of PKD, recognizes the TGN-bound PKD. Thus, activation of intact PKD is important for binding to the TGN (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser744/Ser748 |
| General References: | 1. Johannes FJ, et al. J Biol Chem. 269(8):6140-8.1994. 2. Maeda Y, et al. EMBO J 20(21):5982-90, 2001. 3. Jamora C, et al. Cell 98(1):59-68, 1999. |
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