| request information: |
|
| Catalog Code: | NB110-57357 |
| Product Name: | PKC alpha Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-PKC alpha (pT638) (E195) [Thr638] |
| Clone: | E195 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKCá was used as immunogen. The antibody only detects PKCá phosphorylated on Threonine 638. Predicted to cross-react with bovine and chicken, based on sequence homology. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:20,000IHC: 1:250ICC: 1:500IP: 1:40 |
| Background: | Activation of PKC plays a crucial role in tranducing signals for a variety of cellular responses, including tumor proliferation (1). PKC isoforms have been classified into three groups: classical, novel, and atypical. Members of these three PKC groups contain an autoinhibitory domain that binds to the substrate binding site in the catalytic domain, preventing its activation in the absence of cofactors or activators (2,3). The activity of PKC is under the control of three distinct phosphorylation events, including that of the Thr641 autophosphorylation site (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-AAG6 antibody, anti-MGC129900 antibody, anti-MGC129901 antibody, anti-PICK 1 antibody, anti-PKC A antibody, anti-PKC alpha antibody, anti-PKCA antibody, anti-PRKACA antibody, anti-PRKC A antibody, anti-PRKCA antibody, anti-Protein Kinase C alpha antibody, anti-Protein kinase C alpha type antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Thr638 |
| General References: | 1. Nishizuka, Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 308: 693-698 (1984). 2. Newton, A.C. Protein kinase C: structure, function, and regulation. J. Biol. Chem. 270: 28495-28498 (1995). 3. Mellor, H. and P. Perker. The extended protein kinase C superfamily. Biochem. J. 332: 281-292 (1998). 4. Keranen, L., et al. Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Curr. Biol. 5: 1394-1403 (1995). |
order or
more information: | company product webpage |
|