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| Catalog Code: | NB110-57356 |
| Product Name: | PKC alpha Antibody |
| Product Description: | Rabbit Monoclonal anti-PKC alpha (Y124) |
| Clone: | Y124 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human PKCá was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:5,000-10,000IHC: 1:250-500ICC: 1:250-500Flow Cytometry: 1:100IP: 1:100 |
| Background: | Activation of PKC plays a crucial role in transducing signals for a variety of cellular responses, including tumor proliferation (1). PKC isoforms have been classified into three groups: classical, novel, and atypical. Members of these three PKC groups contain an autoinhibitory domain that binds to the substrate binding site in the catalytic domain, preventing its activation in the absence of cofactors or activators (2,3). The activity of PKC is under the control of three distinct phosphorylation events, including that of the Thr641 autophosphorylation site (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-AAG6 antibody, anti-MGC129900 antibody, anti-MGC129901 antibody, anti-PICK 1 antibody, anti-PKC A antibody, anti-PKC alpha antibody, anti-PKCA antibody, anti-PRKACA antibody, anti-PRKC A antibody, anti-PRKCA antibody, anti-Protein Kinase C alpha antibody, anti-Protein kinase C alpha type antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Nishizuka, Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 308: 693-698 (1984). 2. Newton, A.C. Protein kinase C: structure, function, and regulation. J. Biol. Chem. 270: 28495-28498 (1995). 3. Mellor, H. and P. Perker. The extended protein kinase C superfamily. Biochem. J. 332: 281-292 (1998). 4. Keranen, L., et al. Protein kinase C is regulated in vivo by three functionally distinct phosphorylations. Curr. Biol. 5: 1394-1403 (1995). |
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