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| Catalog Code: | NB110-57351 |
| Product Name: | PKA RII Antibody |
| Product Description: | Rabbit Monoclonal anti-PKA RII (Y116) |
| Clone: | Y116 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the C-terminus of human cAMP-dependent protein kinase type II-alpha regulatory subunit was used as immunogen. The antibody does not cross-react with other cAMP-dependent kinase. |
| Cross Reactivity: | Reacts in human and mouse. Does not react in rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:10,000-50,000IHC: 1:100-250ICC: 1:100-250IP: 1:50 |
| Background: | PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-cAMP dependent protein kinase regulatory subunit alpha 2 antibody, anti-cAMP dependent protein kinase regulatory subunit RII alpha antibody, anti-cAMP dependent protein kinase type II alpha regulatory chain antibody, anti-cAMP dependent protein kinase type II alpha regulatory subunit antibody, anti-MGC3606 antibody, anti-PKR 2 antibody, anti PKR2 antibody, anti-PRKA R2 antibody, anti-PRKAR 2 antibody, anti-PRKAR2 antibody, anti-PRKAR2A antibody, anti-Protein kinase A RII alpha subunit antibody, anti-Protein kinase cAMP dependent regulatory type II alpha antibody |
| Package Size: | 0.1 ml |
| Preservative: | .01% sodium azide |
| General References: | 1. Montminy, M. Transcriptional regulation by cyclic AMP. Annu. Rev. Biochem. 66: 807-822 (1997). 2. Beebe, S.J. The cAMP-dependent protein kinases and cAMP signal transduction. Semin Cancer Biol. 5: 285-94 (1994). 3. Braun, R.K, et al. Phosphorylation of RII subunit and attenuation of cAMP-dependent protein kinase activity by proline-directed protein kinase. Arch Biochem Biophys. 289: 187-91 (1991). |
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