| CatalogCode: | NB110-57344 |
| ProductName: | PDK1 Antibody |
| Product Description: | Rabbit Monoclonal anti-PDK1 (Y336) |
| Clone: | Y336 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide corresponding to residues in the protein kinase region of human PDK1 |
| Specificity: | A synthetic peptide corresponding to residues in the protein kinase region of human PDK1 was used as an immunogen. This antibody is predicted to detect splice isoform 2 based on sequence homology. |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1: 2,000IHC: 1:50-100ICC: 1:250-500 |
| Background: | 3-Phosphoinositide-dependent protein kinase-1 (PDK1) is a serine /threonine protein kinase known to phosphorylate and activate PKB/AKT (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P3 and PtdIns(3,4)P2.,which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1-2). It has also been shown that stimulation of cells with pervanadate and IGF-1 results in a significant increase in PDK1 activity and translocation to the plasma membrane along with an increase in tyrosine phosphorylation (3). Only phosphorylation on the activation loop at Ser241 is necessary for PDK1's activity and a single nucleotide mutation abolishes its activity (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.5% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-3 Phosphoinositide Dependent Protein Kinase 1 antibody, anti-hPDK 1 antibody, anti-hPDK1 antibody, anti-MGC20087 antibody, anti-MGC35290 antibody, anti-PDK 1 antibody, anti-PDPK 1 antibody, anti-PDPK1 antibody, anti-PkB kinase antibody, anti-PkB kinase like gene 1 antibody, anti-PkB like 1 antibody, anti-PkB like antibody, anti-PRO0461 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Alessi et al. Curr. Biol. 7:776-789, 1997 2. Stokoe et al. Science277, 567-570, 1997 3. Jongsun Park, Michelle M. Hill, Daniel hess, Derek P. Brazil, Jan Hofsteenge, and Brian A. Hemmings; J. Biol. Chem., Vol. 276, Issue 40, 37459-37471, October 5, 2001 4. Casamayor, A., Morrice, N.A., and Alessi, D. R (1999) Biochem. J. 342, 287-292 |
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