| CatalogCode: | NB110-57342 |
| ProductName: | Phospho Paxillin (pY31) Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho Paxillin (pY31) (E228) [Tyr31] |
| Clone: | E228 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 31. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:10,000IHC: 1:100-1:250ICC: 1:250IP: 1:100 |
| Background: | Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | # FLJ16691 antibody # FLJ23042 antibody # Paired box protein Pax 1 antibody # PAX 1 antibody # PAX1 antibody # Paxillin alpha antibody # PXN antibody # PXN protein antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Salgia, R., et al. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J Biol Chem. 270: 5039-47 (1995). 2. Burridge, K., et al. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119: 893-903 (1992). 3. Bellis, S.L., et al. Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase. J. Biol. Chem. 270: 17437-17441 (1995). 4. Iwasaki, T., et al. Involvement of phosphorylation of Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration. Int J Cancer. 97: 330-5 (2002). |
| Phosphorylated: | Tyr31 |
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