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| Catalog Code: | NB110-57323 |
| Product Name: | PARP-1 Antibody |
| Product Description: | Rabbit Monoclonal anti-PARP-1 (E102) |
| Clone: | E102 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to N-terminal residues of human PARP-1 was used as immunogen. The antibody should recognize both pro-form and p25 cleaved-form of PARP-1. |
| Cross Reactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000IHC: 1:25ICC: 1:100Flow Cytometry: 1:50 |
| Background: | Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-ADP ribosyltransferase NAD+ antibody, anti-ADPRT antibody, anti-ADPRT1 antibody, anti-NAD(+) ADP ribosyltransferase 1 antibody, anti-pADPRT 1 antibody, anti-pADPRT1 antibody, anti-PARP 1 antibody, anti-PARP1 antibody, anti-Poly adenosine diphosphate ADP ribose polymerase antibody, anti-Poly ADP ribose polymerase 1 antibody, anti-Poly ADP ribose polymerase family member 1 antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-PPOL antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Ikejima, M, et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J. Biol. Chem. 265: 21907-13 (1990). 2. Lazebnik, Y.A., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371: 346-347 (1994). 3. Tewari, M, et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801-9 (1995). 4. Kaufmann, S.H., et al. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res. 53: 2976-3985 (1993) |
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