| CatalogCode: | | NB110-57321 |
| ProductName: | | PARP-1 (Cleaved p85) Antibody |
| Product Description: | | Rabbit Monoclonal anti-PARP-1 (Cleaved p85) (Y34) |
| Clone: | | Y34 |
| Clonality: | | Monoclonal |
| Specificity: | | A synthetic peptide corresponding to residues following the cleavage site of human PARP-1 was used as immunogen. The antibody only recognize p85 cleaved-form of PARP-1. |
| CrossReactivity: | | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | | 0.1 ml Rabbit ascites. |
| Uses: | | WB: 1:1,000ICC: 1:50Flow Cytometry: 1:50IP: 1:50 |
| Background: | | Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa and 85 kDa (2, 4). |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.05% BSA. |
| ListPrice: | | 325 |
| AppSummary: | | FACS, IP, WB, ICC |
| SpeciesSummary: | | Hu, Mu(-), Rt |
| ALTnames: | | ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody NAD + ADP ribosyltransferase 1 antibody pADPRT 1 antibody pADPRT1 antibody PARP 1 antibody PARP antibody PARP1 antibody Poly ADP ribose polymerase 1 antibody Poly ADP ribose polymerase antibody Poly ADP ribose polymerase family member 1 antibody Poly ADP ribose synthetase 1 antibody Poly ADP ribose synthetase antibody Poly ADP ribosyl transferase antibody PPOL antibody |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Ikejima, M, et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J. Biol. Chem. 265: 21907-13 (1990). 2. Lazebnik, Y.A., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371: 346-347 (1994). 3. Tewari, M, et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801-9 (1995). 4. Kaufmann, S.H., et al. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res. 53: 2976-3985 (1993). |
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