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Rabbit Monoclonal anti-p73 (EP436Y)
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CatalogCode:NB110-57313
ProductName:p73 Antibody
Product Description:Rabbit Monoclonal anti-p73 (EP436Y)
Clone:EP436Y
Clonality:Monoclonal
Specificity:A synthetic peptide corresponding to residues near the transactivation domain of human p73 was used as immunogen. p73 has a predicted molecular weight of 73 kDa but is detected at 63kDa.
CrossReactivity:Reacts in human, mouse, and rat. Not tested in other species.
Packaging:0.1 ml
Uses:WB: 1:500-2,000IHC: 1:100-250ICC: 1:150IP: 1:50
Background:p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over- expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (á, â, and ã) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).
Storage:Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype:IgG
Host_Name:Rabbit
Buffer:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
ListPrice:325
AppSummary:IHC, IP, WB, ICC
SpeciesSummary:Hu, Mu, Rt
ALTnames:p53 like transcription factor antibody p53 Related Protein antibody p73 antibody TP 73 antibody TP73 antibody Tumor Protein p73 antibody
PackageSize:0.1 ml
GeneralRef:1. Kaghad et al. Cell 90: 809-819, 1997. 2. Yang, A., Kaghad, M., Wang, Y., Gillett, E., Fleming, M. D., Dotsch, V., Andrews, N. C., Caput, D., and McKeon, F. (1998) Mol. Cell 2, 305-316 3. Marin MC, Jost CA, DeCaprio JA, Caput D, Kaelin WG Jr. Mol Cell Biol 1998;18:6316-24 4. Levrero, M., De Laurenzi, V., Costanzo, A., Gong, J., Melino, G., and Wang, J. Y. (1999) Cell Death Differ. 6, 1146-1153 5. Marin et al. Nature Genet. 25: 47-54, 2000.
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