| CatalogCode: | NB110-57311 |
| ProductName: | Phospho-p70 S6 Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-p70 S6 (E175) [Thr389] |
| Clone: | E175 |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic phospho-peptide corresponding to residues surrounding Thr389 of human p70 S6 kinase |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Thr389 of human p70 S6 kinase was used as immunogen. The antibody only detects p70 S6 kinase phosphorylated on Threonine 389. Predicted to cross-react with bovine, based on sequence homology. |
| CrossReactivity: | Reacts in human and mouse. Does not react in rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody works for IHC, IP, WB, and is unsuitable for FACS or ICC. |
| Localization: | Cytoplasmic |
| Control: | Jurkat cell lysate; human breast carcinoma |
| Background: | p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB |
| SpeciesSummary: | Hu, Mu, Rt(-) |
| ALTnames: | anti-0 kDa ribosomal protein S6 kinase 1 antibody; anti-p70 alpha antibody; anti-P70 beta 1 antibody; anti-p70 ribosomal S6 kinase beta 1 antibody; anti-p70 S6 kinase alpha antibody; anti-P70 S6 Kinase antibody; anti-p70 S6K antibody; anti-p70(S6K) alpha antibody; anti-P70S6K antibody; anti-p70s6kA antibody; anti-p70S6Kb antibody; anti-PS6K antibody; anti-Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody; anti-Ribosomal protein S6 kinase 70kDa polypeptide 2 antibody; anti-Ribosomal protein S6 kinase beta 1 antibody; anti-Ribosomal protein S6 kinase I antibody; anti-RPS6KB1 antibody; anti-RPS6KB2 antibody; anti-S6K antibody; anti-S6K1 antibody; anti-Serine/threonine kinase 14 alpha antibody; anti-STK14A antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Pearson, R.B. and G. Thomas. Regulation of p70s6k/p85s6k and its role in the cell cycle. Prog Cell Cycle Res. 1: 21-32 (1995). 2. Pullen, N. and G. Thomas. The modular phosphorylation and activation of p70s6k. FEBS Lett. 410: 78-82 (1997). 3. Dufner, A. and G. Thomas. Ribosomal S6 kinase signaling and the control of translation. Exp Cell Res. 253: 100-9 (1999). 4. Le, X.F., et al. Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis. Oncogene 22: 484-97 (2003). |
| Phosphorylated: | Thr389 |
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more information: | company product webpage |
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