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Rabbit Monoclonal anti-Ras/p21 (Y131)
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CatalogCode:NB110-57292
ProductName:Ras/p21 Antibody
Product Description:Rabbit Monoclonal anti-Ras/p21 (Y131)
Clone:Y131
Clonality:Monoclonal
Specificity:A synthetic peptide corresponding to residues in the effector region of human Ras was used as an immunogen. Predicted to react with other Ras family members and cross-react with most species based on sequence homology.
CrossReactivity:Reacts in human. Does not react in mouse or rat. Not tested in other species.
Packaging:0.1 ml Rabbit ascites.
Uses:WB: 1:500-1,000ICC: 1:50
Background:Ras, a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4).
Storage:Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype:IgG
Host_Name:Rabbit
Buffer:50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
ListPrice:325
AppSummary:WB, ICC
SpeciesSummary:Hu, Mu(-), Rt(-)
ALTnames:C K RAS antibody c K ras protein antibody c K ras2 protein antibody c Ki ras antibody C Kirsten ras protein antibody Cellular c Ki ras2 proto oncogene antibody GTPase KRas antibody GTPase NRas antibody K Ras 2 antibody K ras p21 protein antibody K RAS2A antibody K RAS2B antibody K RAS4A antibody K RAS4B antibody KI RAS antibody Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog antibody KRAS antibody KRAS1 antibody KRAS2 antibody N ras antibody Neuroblastoma RAS viral (v-ras) oncogene homolog antibody NRAS antibody NRAS1 antibody NS3 antibody oncogene KRAS2 antibody PR310 c K ras oncogene antibody RASK2 antibody Transforming protein N Ras antibody transforming protein p21 antibody v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody v ras neuroblastoma RAS viral oncogene homolog antibody
PackageSize:0.1 ml
GeneralRef:1. Ellis RW, et al. Nature. 1981 Aug 6;292(5823):506-511 2. Campbell, S. L., (1998) Oncogene 17, 1395-1413 3. Zheng, Y., and Quilliam, L. A. (2003) EMBO Rep 4, 463-468 4. Bos JL. Cancer Res. 1989;49:4682-4689
see all human HRAS antibodies
see anti rabbit secondary antibodies


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