| request information: |
|
| Catalog Code: | NB110-57291 |
| Product Name: | p190-B RhoGAP Antibody |
| Product Description: | Rabbit Monoclonal anti-p190-B RhoGAP (EP489Y) |
| Clone: | EP489Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Recombinant full length protein (Human) of p16/INK4a |
| Specificity: | A synthetic peptide corresponding to residues in human p190-B RhoGAP was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1: 500IHC: 1:100-250Flow Cytometry: 1: 60IP: 1: 50 |
| Background: | The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.5% BSA. |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry , immunoprecipitation , Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-ARF antibody, anti-CCM2 antibody, anti-CDK4 Inhibitor antibody, anti-CDK4I antibody, anti-CDKN2 antibody, anti-CDKN2A antibody, anti-CMM2 antibody, anti-Cyclin dependent kinase 4 inhibitor A antibody, anti-Cyclin Dependent Kinase Inhibitor 2A antibody, anti-Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody, anti-INK4 antibody, anti-INK4A antibody, anti-MLM antibody, anti-MTS1 antibody, anti-Multiple Tumor Supressor 1 antibody, anti-p14 antibody, anti-p14(ARF) antibody, anti-p14ARF antibody, anti-p16 antibody, anti-p16 INK4 antibody, anti-p16INK4 antibody, anti-p16INK4a antibody, anti-p19 antibody, anti-p19(ARF) antibody, anti-TP16 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Burbelo et al. J. Biol. Chem. 270: 30919-30926, 1995. 2. Peter et al. Volume 270, Number 52, Issue of December 29, 1995 pp. 30919-30926 3. DZ Wang, MS Nur-E-Kamal, A Tikoo, W Montague and H Maruta. Cancer Research, Vol 57, Issue 12 2478-2484. |
| Novus References: | 1. Rosman DS, Phukan S, Huang C-C, Pasche B. TGFBR1*6A Enhances the Migration and Invasion of MCF-7 Breast Cancer Cells through RhoA Activation. Cancer Res. March 1, 2008;68(5):1319-28. |
order or
more information: | company product webpage |
|