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| Catalog Code: | NB110-57279 |
| Product Name: | Akt1/PKBa Antibody |
| Product Description: | Rabbit Monoclonal anti-Akt1/PKBa (E45) |
| Clone: | E45 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Specificity: | A synthetic peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody can detects Akt1 and Akt3. Predicted to cross-react with bovine based on sequence homology |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | IHC: 1:1,000-2,500ICC: 1:1,000IP: 1:100 |
| Background: | Akt1/PKBá is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBá is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBá inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBá regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBá isoforms have been identified. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-C AKT antibody, anti-MGC9965 antibody, anti-Oncogene AKT1 antibody, anti-PKB antibody, anti-PRKBA antibody, anti-Protein Kinase B Alpha antibody, anti-RAC Alpha antibody, anti-RAC antibody, anti-RAC PK Alpha antibody, anti-RAC Serine/Threonine Protein Kinase antibody, anti-vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Burgering, B.M. and P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599-602 (1995). 2. Cardone, M.H., et al. Regulation of cell death protease caspase-9 by phosphorylation. Science 282: 1318-1321 (1998). 3. Hajduch, E., et al. Protein kinase B (PKB/Akt)--a key regulator of glucose transport? FEBS Lett. 492: 199-203 (2001). 4. Cross, D.A., et al. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 378: 785-789 (1995). |
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