| CatalogCode: | NB110-57267 |
| ProductName: | NF-kB p65 Antibody |
| Product Description: | Rabbit Monoclonal anti-NF-kB p65 (E379) |
| Clone: | E379 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human nuclear factor NF-kappa-B p65 subunit was used as immunogen. |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, and Immunoprecipitation. |
| Background: | Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB. (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IęB family, retains NF-ęB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (4). In addition to the nuclear translocation and DNA binding, NF-B's transcriptional activity is also regulated by coactivators and corepressors in the nucleus. Interaction of p65 with coactivators; CREB-binding protein and p300, increases its transcriptional activity (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | MGC131774 antibody NFKB 3 antibody NFKB3 antibody Nuclear Factor NF Kappa B p65 Subunit antibody Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody RELA antibody Transcription Factor p65 antibody V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody v-rel reticuloendotheliosis viral oncogene homolog A antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Baldwin, A. S., Jr. (1996) Annu. Rev. Immunol. 14, 649-683 2. Verma, I., Stevenson, J., Schwarz, E., Van Antwerp, D. & Miyamoto, S. (1995) Genes Dev. 9, 2723-2735 3. Schmitz, M., dos Santos Silva, M. & Baeuerle, P. (1995) J. Biol. Chem. 270, 15576-15584 4. Marc D. Jacobs Stephen C. Harrison (1198) Cell. Volume 95, Issue 6 , 749-758 |
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