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| Catalog Code: | NB110-57266 |
| Product Name: | NF-kB p105/50 Antibody |
| Product Description: | Rabbit Monoclonal anti-NF-kB p105/50 (E381) |
| Clone: | E381 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | A synthetic peptide corresponding to residues in the nuclear factor NFkB p50 subunit between amino acids 325-375. |
| Specificity: | A synthetic peptide corresponding to residues in the nuclear factor NF-êB p50 subunit was used as immunogen. This antibody will detect both forms; p50 and p105. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:5,000IHC: 1:250-500ICC: 1:250-500Flow Cytometry: 1: 30 |
| Background: | Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). The family members include p50, p52, p65 (RelA), c-Rel, and RelB (2). Association with inhibitory proteins of the IêB family retains NF-êB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA (3). The p105 precursor protein acts as an NFêB inhibitor, retaining Rel subunits in the cytoplasm of unstimulated cells. Upon stimulation by TNFá and IL -1á, degradation of p105 occurs; the Rel subunit is released and translocats to the nucleus (4). Additionally, p105 will be proteolytically processed by the 26S proteasome to produce a smaller, transcriptionally active form (p50) (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry , Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-DKFZp686C01211 antibody, anti-DNA binding factor KBF1 antibody, anti-DNA binding factor KBF1 EBP1 antibody, anti-EBP 1 antibody, anti-EBP1 antibody, anti-KBF1 antibody, anti-MGC54151 antibody, anti-NF kappa B antibody, anti-NFKB 1 antibody, anti-NFKB p105 antibody, anti-NFKB p50 antibody, anti-NFKB1 antibody, anti-Nuclear factor kappa B DNA binding subunit antibody, anti-Nuclear factor NF kappa B p105 subunit antibody, anti-Nuclear factor NF kappa B p50 subunit antibody, anti-Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Baldwin, A. S., Jr. (1996) Annu. Rev. Immunol. 14, 649-683 2. Verma, I., Stevenson, J., Schwarz, E., Van Antwerp, D. & Miyamoto, S. (1995) Genes Dev. 9, 2723-2735 3. Marc D. Jacobs Stephen C. Harrison. (1998); Cell Volume 95, Issue 6, 749-758 4. Rice, N. R., MacKichan, M. L., and Israel, A. (1992) Cell 71, 243-253 5. Palombella, V. J., Rando, O. J., Goldberg, A. L., and Maniatis, T. (1994) Cell 78, 773-785 |
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