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| Catalog Code: | NB110-57263 |
| Product Name: | Heparanase 1 Antibody |
| Product Description: | Mouse Monoclonal anti-Heparanase 1 (HP3/17) |
| Clone: | HP3/17 |
| Clonality: | Monoclonal |
| ListPrice: | 360 |
| Concentration: | 4 mg/ml |
| Immunogen: | Raised against a polypeptide from the 50 kDa subunit of Heparanase |
| Specificity: | HP3/17 reacts with the 50 kDa subunit and with the 65 kDa precursor of human or mouse Heparanase by Western blotting and immunohistochemistry. |
| Cross Reactivity: | Human and Mouse. Not tested in any other species. |
| Packaging: | 0.05 mg protein G purified Mouse ascites. |
| Background: | Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasations of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and an 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2BK |
| Host Name: | Mouse |
| Buffer: | 20 mM Sodium Phosphate, 150 mM NaCl, pH 7.2 |
| Application Summary: | ELISA , immunohistochemistry 1/250, Western Blot 1/4000 |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-HPSE1 antibody, anti-HPA1 antibody, anti-HPA-1 antibody, anti-HPR1 antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.001 Thimerosal |
| General References: | 1. Enomoto K, et al. A simple and rapid assay for heparanase activity using homogeneous time-resolved fluorescence. J. Pharm. Biomed. Anal. Jun 2006; 41(3): 912-917. 2. Baraz L, et al. Tumor suppressor p53 regulates heparanase gene expression. Oncogene. Jun 2006; 25(28): 3939-3947. |
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