| CatalogCode: | NB110-57227 |
| ProductName: | MSK1 Phospho (pS376) Antibody |
| Product Description: | Rabbit Monoclonal anti-MSK1 Phospho (pS376) (E375) |
| Clone: | E375 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Serine 376 of human MSK1 was used as immunogen. The antibody will detect MSK1 phosphorylation on Serine 376. |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000-5,000IHC: 1:50-100ICC: 1:100-250IP: 1:30 |
| Background: | MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | 90 kDa Ribosomal Protein S6 Kinase 5 antibody MSPK1 antibody Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody Ribosomal Protein S6 Kinase Alpha 5 antibody RLPK antibody RPS6KA5 antibody RSK Like Protein Kinase antibody RSKL antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Deak M, Clifton AD, Lucocq LM, Alessi DR, EMBO J 1998;17:4426-41 2. Pierrat et al. (1998) J.Biol. Chem. 273, 29661 3. New et al (1999) J. Biol. Chem. 274, 1026-18852 4. Claire E. McCoy et al. Biochemical Journal Immediate Publication.November 29, 2004 |
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