| request information: |
|
| Catalog Code: | NB110-57210 |
| Product Name: | MLK-3 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-MLK-3 (C-term) (EP1460Y) |
| Clone: | EP1460Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues on the C-terminus of human MLK-3 was used as an immunogen |
| Cross Reactivity: | Reacts in human and mouse. Does not react in rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:5000ICC: 1:100 - 250FC: 1:15 |
| Background: | Mixed lineage kinase-3 (MLK-3) is a mitogen-activated kinase kinase kinase that mediates stress-activating protein kinase (SAPK)/c-Jun NH2-terminal kinase activation. MLK-3 and other MLK family kinases are characterized by the presence of multiple protein-protein interaction domains including a tandem leucine/isoleucine zipper (LZs) motif. Leucine zippers are known to mediate protein dimerization raising the possibility that the tandem leucine/isoleucine zippers may function as a dimerization motif of MLK-3 (1). MLK-3 has several interesting structural features including an SH3 domain in the absence of an SH2 domain, a region containing two leucine zippers with an adjacent carboxy-terminal basic region, and a proline rich region. (2). HPK1 has been found to phosphorylate MLK-3 activation loop in vitro, and Ser281 was found to be the major phosphorylation site, indicating that HPK1 also activates MLK-3 via phosphorylation of the kinase activation loop (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt(-), |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Leung IW, et al. J Biol Chem 273(49):32408-15, 1998. 2. Ing YL, et al. Oncogene 9(6):1745-50, 1994. 3. Leung IW, et al. J Biol Chem 276(3):1961-7, 2001. |
order or
more information: | company product webpage |
|