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| Catalog Code: | NB110-57192 |
| Product Name: | MEK2 (N-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-MEK2 (N-term) (Y78) |
| Clone: | Y78 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues in N terminus of human MEK2 |
| Specificity: | A synthetic peptide corresponding to residues in N-terminus of human MEK2 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members. |
| Cross Reactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:10,000IHC: 1:250-1:500ICC: 1:250-1:500Flow Cytometry: 1:40IP: 1:100 |
| Background: | MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry , immunoprecipitation , Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-Cardiofaciocutaneous syndrome antibody, anti-CFC syndrome antibody, anti-Dual specificity mitogen activated anti-protein kinase kinase 2 antibody, anti-ERK activator kinase 2 antibody, anti-MAP kinase kinase 2 antibody, anti-MAP2K 2 antibody, anti-MAPK / ERK kinase 2 antibody, anti-MAPKK 2 antibody, anti-MAPKK2 antibody, anti-MEK 2 antibody, anti-Microtubule Associated Protein Kinase Kinase 2 antibody, anti-Mitogen activated protein kinase kinase 2 p45 antibody, anti-MKK 2 antibody, anti-MKK2 antibody, anti-PRKMK 2 antibody, anti-PRKMK2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Crews, C. M., et al. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258: 478-480 (1992). 2. Zheng, C.F., and K.L. Guan. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268: 11435-9 (1993). 3. Zheng, C.F., and K.L. Guan. Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 13: 1123-31 (1994). 4. Alessi, D.R., et al. Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J. 13: 1610-1619 (1994). 5. Rosen, L.B., et al. Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras. Neuron 12: 1207-1221 (1994). |
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