| CatalogCode: | | NB110-57191 |
| ProductName: | | Phospho MEK1 (pS218/222) Antibody |
| Product Description: | | Rabbit Monoclonal anti-Phospho MEK1 (pS218/222) (E237) [Ser218/Ser222] |
| Clone: | | E237 |
| Clonality: | | Monoclonal |
| Specificity: | | A synthetic phospho-peptide corresponding to residues surrounding Ser218 and Ser222 of human MEK1 was used as immunogen. The antibody only detects MEK1 phosphorylated on Serine 218 and 222. |
| CrossReactivity: | | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | | 0.1 ml |
| Uses: | | WB: 1:1000IHC: 1:50-100ICC: 1:50-100IP: 1:30 |
| Background: | | MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5). |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | | 325 |
| AppSummary: | | IHC, IP, WB, ICC |
| SpeciesSummary: | | Hu, Mu(-), Rt(-) |
| ALTnames: | | Dual specificity mitogen activated protein kinase kinase 1 antibody ERK activator kinase 1 antibody MAP kinase kinase 1 antibody MAP kinase/Erk kinase 1 antibody MAP/Erk kinase 1 antibody Map2K1 antibody MAPKK 1 antibody MAPKK1 antibody MEK 1 antibody MEKK1 antibody Mitogen activated protein kinase kinase 1 antibody MKK 1 antibody MKK1 antibody PRKMK 1 antibody PRKMK1 antibody Protein kinase mitogen activated kinase 1 antibody |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Crews, C. M., et al. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258: 478-480 (1992). 2. Zheng, C.F., and K.L. Guan. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268: 11435-9 (1993). 3. Zheng, C.F., and K.L. Guan. Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 13: 1123-31 (1994). 4. Alessi, D.R., et al. Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J. 13: 1610-1619 (1994). 5. Rosen, L.B., et al. Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras. Neuron 12: 1207-1221 (1994). |
| Phosphorylated: | | Ser218/Ser222 |
| more information: | | company product webpage |
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