| CatalogCode: | | NB110-57189 |
| ProductName: | | MEK1 (C-term) Antibody |
| Product Description: | | Rabbit Monoclonal anti-MEK1 (C-term) (Y77) |
| Clone: | | Y77 |
| Clonality: | | Monoclonal |
| Immunogen: | | Synthetic peptide, corresponding to residues in the C terminus of human MEK1. |
| Specificity: | | A synthetic peptide corresponding to residues in C-terminus of human MEK1 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members. |
| CrossReactivity: | | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | | 0.1 ml Rabbit ascites. |
| Uses: | | WB: 1:10,000IHC: 1:250ICC: 1:250-1:500Flow Cytometry: 1:40IP: 1:50 |
| Background: | | MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5). |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Ascites |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | | 325 |
| AppSummary: | | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | | Hu, Mu(-), Rt |
| ALTnames: | | anti-Dual specificity mitogen activated anti-protein kinase kinase 1 antibody, anti-ERK activator kinase 1 antibody, anti-MAP kinase kinase 1 antibody, anti-MAP kinase/Erk kinase 1 antibody, anti-MAP/Erk kinase 1 antibody, anti-Map2K1 antibody, anti-MAPKK 1 antibody, anti-MAPKK1 antibody, anti-MEK 1 antibody, anti-MEKK1 antibody, anti-Mitogen activated protein kinase kinase 1 antibody, anti-MKK 1 antibody, anti-MKK1 antibody, anti-PRKMK 1 antibody, anti-PRKMK1 antibody, anti-Protein kinase mitogen activated kinase 1 antibody |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Crews, C. M., et al. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258: 478-480 (1992). 2. Zheng, C.F., and K.L. Guan. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268: 11435-9 (1993). 3. Zheng, C.F., and K.L. Guan. Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues. EMBO J. 13: 1123-31 (1994). 4. Alessi, D.R., et al. Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1. EMBO J. 13: 1610-1619 (1994). 5. Rosen, L.B., et al. Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras. Neuron 12: 1207-1221 (1994). |
| more information: | | company product webpage |
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