| CatalogCode: | NB110-57183 |
| ProductName: | MARCKS Antibody |
| Product Description: | Rabbit Monoclonal anti-MARCKS (EP1446Y) |
| Clone: | EP1446Y |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic peptide corresponding to residues on human MARCKS. |
| Specificity: | A synthetic peptide corresponding to residues on human MARCKS was used as an immunogen. |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | WB: 1:10,000IHC: 1:100 - 250ICC: 1:100 - 250FC: 1:10 |
| Background: | The expression of a major cellular substrate for protein kinase C, the MARCKS protein, is regulated in a cell-, tissue-, and developmental stage-specific fashion; in addition, this expression can be stimulated acutely by various cytokines in certain cell types (1). MARCKS) binds calmodulin and is a predominant, specific substrate of PKC which is phosphorylated during macrophage and neutrophil activation, growth factor-dependent mitogenesis and neurosecretion; it is redistributed from plasma membrane to cytoplasm when phosphorylated and is involved in leukocyte motility. It has been reported that MARCKS is a filamentous (F) actin crosslinking protein, with activity that is inhibited by PKC-mediated phosphorylation and by binding to calcium-calmodulin. MARCKS may be a regulated crossbridge between actin and the plasma membrane, and modulation of the actin crosslinking activity of the MARCKS protein by calmodulin and phosphorylation represents a potential convergence of the calcium-calmodulin and PKC signal transduction pathways in the regulation of the actin cytoskeleton (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | anti-80K L protein antibody, anti-80KL antibody, anti-81 kDa protein, light chain antibody, anti-MACS antibody, anti-MARCS antibody, anti-MGC52672 antibody, anti-Myristoylated alanine rich protein kinase C substrate antibody, anti-Phosphomyristin antibody, anti-PKCSL antibody, anti-PRKCSL antibody, anti-Protein kinase C substrate antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Harlan DM, et al. J Biol Chem 266(22):14399-405, 1991 2. Hartwig JH, et al. Nature 356(6370):618-22, 1992. |
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