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| Catalog Code: | NB110-57157 |
| Product Name: | LAT Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-LAT (pY191) (E225) [Tyr191] |
| Clone: | E225 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Tyr191 of human LAT was used as immunogen. The antibody only detects?n LAT phosphorylated on Tyrosine 191. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2,000IHC: 1:50ICC: 1:50IP: 1:10 |
| Background: | LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-E2(25K) antibody, anti-Huntingtin interacting protein 2 antibody, anti-UBC1 antibody, anti-Ubiquitin carrier protein antibody, anti-Ubiquitin conjugating enzyme E2 25 kDa antibody, anti-Ubiquitin protein ligase antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Tyr191 |
| General References: | 1. Weber, J.R., et al. Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells. J Exp Med. 187: 1157¡V61 (1998). 2. Zhang, W., et al. LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. Cell 92: 83¡V92 (1998). 3. Paz, P.E., et al. Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells. Biochem J. 356(Pt 2): 461¡V71 (2001). |
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