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| Catalog Code: | NB110-57156 |
| Product Name: | LAT Antibody |
| Product Description: | Rabbit Monoclonal anti-LAT (Y109) |
| Clone: | Y109 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues surrounding GRB2-binding motif 2 of human LAT was used as immunogen. This antibody detects both long and short forms of LAT. |
| Cross Reactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500IHC: 1:100Flow Cytometry: 1:40IP: 1:60 |
| Background: | LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-36 kDa phospho tyrosine adapter protein antibody,anti-T 1 antibody, anti-LAT1 antibody, anti-nker for activation of T cells antibody, anti-nker for activation of T cells family member 1 antibody, anti-6 38 antibody, anti-36 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Weber, J.R., et al. Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells. J Exp Med. 187: 1157-61 (1998). 2. Zhang, W., et al. LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. Cell 92: 83-92 (1998). 3. Paz, P.E., et al. Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells. Biochem J. 356(Pt 2): 461-71 (2001). |
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