| request information: |
|
| Catalog Code: | NB110-57140 |
| Product Name: | IRS-4 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-IRS-4 (C-term) (EP907Y) |
| Clone: | EP907Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues from the C terminus of human IRS4. |
| Specificity: | A synthetic peptide corresponding to residues on the C-terminus of human IRS-4 was used as an immunogen. |
| Cross Reactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | WB: 1:5000IHC: 1:50 - 100FC: 1:20IP: 1:20 |
| Background: | A new member of the IRS family, IRS-4, is a 160-kDa tyrosine kinase which phosphorylates cellular substrates when it is activated by the binding of insulin (1). IRS-4 was originally discovered in human embryonic kidney 293 cells. Subcellular fractionation revealed that about 50% of IRS-4 was located in cellular membranes, and immunofluorescence indicated that IRS-4 was concentrated at the plasma membrane. Immunoelectron microscopy conclusively established that a large portion of the IRS-4 was located at the cytoplasmic surface of the plasma membrane in both the unstimulated and insulin-treated states. IRS-4 was found to be associated with two src homology 2 (SH2) domain-containing proteins, phosphatidylinositol 3-kinase and Grb2, the adaptor to the guanine nucleotide exchange factor for Ras (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry , immunoprecipitation , Western Blot |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-Insulin receptor substrate 4 antibody, anti-PY160 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Lavan BE, J Biol Chem 272(34):21403-7, 1997 2. Fantin VR, et al. 273(17):10726-32, 1998. |
order or
more information: | company product webpage |