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| Catalog Code: | NB110-57125 |
| Product Name: | IPP-1 Antibody |
| Product Description: | Rabbit Monoclonal anti-IPP-1 (EP902Y) |
| Clone: | EP902Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues in the N-terminus of human Protein phosphotase inhibitor 1. |
| Specificity: | A synthetic peptide corresponding to residues in the N-terminus of human IPP-1 was used as an immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Whole Rabbit ascites. |
| Uses: | WB: 1:100,000IHC: 1:100-250FC 1:80IP: 1:50 |
| Background: | The inhibitor of protein phosphatase 1 (IPP-1, I-1) is an endogenous inhibitor of protein phophastase 1 (PP-1), a signal transducer in many cellular pathways. IPP-1 is a member of heat-stable PP1 inhibitory subunits, which includes DARPP-32, (a homologue of IPP-1) and IPP-2. First discovered in rabbit skeletal muscle, IPP-1 is expressed in various mammalian tissues. IPP-1 inhibition of PP-1 is controlled through phosphorylation at Thr35 by cAMP-dependent protein kinase and a tetra-peptide consensus motif in various PP-1 binding proteins (1). IPP-1 is a hormonal control in muscle contraction, pituitary cell growth, synaptic plasticity by neurotransmitters, and glycogen metabolism (2). In vivo, IPP-1 is phosphorylated at Ser-67 by NCLK, which also initiates inhibition of PP-1 by IPP-1 (3). Triggered by increase in calcium level, IPP-1 is inactivated by PP2B through de-phosphorylation (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry , immunoprecipitation , Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Protein phosphatase inhibitor 1 antibody, anti-I1 antibody, anti-IPP1 antibody, anti-PPP1R1A antibody, anti-Protein phosphatase 1 regulatory subunit 1A antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Egloff MD, et al. EMBO J 16:1876-1887, 1997. 2. Oliver CJ, Shenolikar S, Front Biosci 3:D961-972, 1998. 3. Huang K, Paudel HK, PNAS 97:5824-5829, 2000. 4. Castellani GC, Learn Mem 12:423-432, 2005. |
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