| CatalogCode: | NB110-57121 |
| ProductName: | ILK Antibody |
| Product Description: | Rabbit Monoclonal anti-ILK (EP1593Y) |
| Clone: | EP1593Y |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide corresponding to residues near N terminus of human Integrin linked ILK |
| Specificity: | A synthetic peptide corresponding to residues near N-term of human ILK protein was used as immunogen |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | WB: 1:2,000 -1:10,000IHC: 1:50-1:100ICC; 1:100-1:250IP: 1:30 |
| Background: | Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BS |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Boulter E et al. Eur J Cell Biol. 85(3-4):255-63, 2006. 2. Wu C et al. J Cell Biol. 155(4):505-10, 2001. 3. Marotta A et al. Oncogene. 20:6250-6257, 2001. |
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