| CatalogCode: | NB110-57069 |
| ProductName: | Huntingtin Protein (Htt) Antibody |
| Product Description: | Rabbit Monoclonal anti-Huntingtin Protein (Htt) (EP867Y) |
| Clone: | EP867Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues specific to the apopain cleavage site of human huntingtin protein was used as an immunogen |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:10,000IHC: 1:250 - 500ICC: 1:50 - 100FC: 1:30 |
| Background: | Huntingtin protein (Htt) is a 348 kDa protein product of Huntington's disease (HD, IT15) gene. Huntington's disease is a neurodegenerative disorder caused by a mutation on the HD gene, producing a polyglutamin (polyQ) expansion on the N-terminus of Htt. The mutant Htt leads to cytotoxicity in the striatal neuron (1). This cytotoxicity is modulated by proteolytic cleavage with caspases and calpains, producing N-terminal polyQ fragments. A phosphorylation of Htt may also regulate the cleavage and cytotoxicity of mutant Htt (2). The wild-type Htt up-regulates transcription of brain-derived neurotrophic factor (BDNF), a pro-survival factor for striatal neurons. This suggests restoring wild-type Htt level and increasing BDNF expression level can be therapeutic treatment for Huntington's disease (3). Huntingtin is also cleaved by apopain, a cysteine protease product of death-gene for human, suggesting HD might be a disorder of inappropriate apoptosis (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-HD protein antibody, anti-HDH antibody, anti-HTT antibody, anti-HUNTINGTON CHOREA antibody, anti-Huntington disease protein antibody, anti-Huntington's disease protein homolog antibody, anti-IT 15 antibody, anti-IT15 antibody, anti-ZHD antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Mende-Mueller LM, et al. J Neuro 21:1830-1837, 2001. 2. Schilling B, et al. J Biol Chem 281:23686-23697, 2006. 3. Zuccato C, et al. Science 293:445-446, 2001. 4. Goldberg YP, et al Nat Genet 13:442-449, 1996. |
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