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| Catalog Code: | NB110-57067 |
| Product Name: | HtrA2 Antibody |
| Product Description: | Rabbit Monoclonal anti-HtrA2 (E56) |
| Clone: | E56 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to C-terminal residues of human HtrA2 was used as immunogen. The antibody should recognize Isoform 1, 2 and 3 of HtrA2 as the sequence used for immunogen is common to them. The antibody does not cross-react with other peptidase S1C family members. |
| Cross Reactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000IHC: 1:100ICC: 1:100Flow Cytometry: 1:50 |
| Background: | HtrA2 (Omi) is a serine protease that plays a significant role in programmed cell death, or apoptosis. Localized to the intermembrane space of the mitochondria, HtrA2 exists predominantly as two isoforms (1). In response to cellular stress signals, HtrA2 is released into the cytosol. One way that HtrA2 induces apoptosis is by binding to BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), and thereby inhibiting their caspase-inhibitory activity (2,3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-HtrA like serine protease antibody, anti-HtrA serine peptidase 2 antibody, anti-Omi stress regulated endoprotease antibody, anti-PARK 13 antibody, anti-PARK13 antibody, anti-Protease serine 25 antibody, anti-PRSS 25 antibody, anti-PRSS25 antibody, anti-Serine protease 25 antibody, anti-Serine protease HTRA2 mitochondrial antibody, anti-Serine protease htra2 mitochondrial precursor antibody, anti-Serine protease omi antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Gray, C.W. et al. Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response. Eur. J. Biochem. 267: 5699-5710 (2000). 2. Savopoulos, J.W. et al. Expression, purification, and functional analysis of the human serine protease HtrA2. Protein Expr. Purif. 19: 227-234 (2000). 3. Suzuki, Y. et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 8: 613-21 (2001). |
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