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| Catalog Code: | NB110-57065 |
| Product Name: | Hsp73/HSPA8 Antibody |
| Product Description: | Rabbit Monoclonal anti-Hsp73/HSPA8 (EP1531Y) |
| Clone: | EP1531Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues near the C terminus of human Hsc70. |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human Hsp73 was used as an immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500IHC: 1:250 - 500ICC: 1:100 - 250FC: 1:30IP: 1:10 |
| Background: | Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry , immunoprecipitation , Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Heat shock 70kD protein 10 antibody, anti-Heat shock 70kD protein 8 antibody, anti-Heat shock 70kDa protein 8 antibody, anti-Heat shock cognate 71 kDa protein antibody, anti-Heat shock cognate protein 54 antibody, anti-Heat shock cognate protein 71 kDa antibody, anti-HSC54 antibody, anti-HSC71 antibody, anti-HSP71 antibody, anti-HSP73 antibody, anti-HSPA10 antibody, anti-HSPA8 antibody, anti-LAP1 antibody, anti-Lipopolysaccharide associated protein 1 antibody, anti-LPS associated protein antibody, anti-MGC29929 antibody, anti-NIP71 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Ungewickell E, EMBO J. 4(13A):3385-91, 1985. 2. Matsui H, et al. Mol Cell. 25(1):99-112, 2007. 3. Deichmann M, et al. Int J Oncol 25(2):259-68, 2004. |
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