| CatalogCode: | NB110-57062 |
| ProductName: | Phospho-HSP27 (pS82) Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-HSP27 (pS82) (E118) [Ser82] |
| Clone: | E118 |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic phospho-peptide corresponding to residues surrounding Ser82 of human Hsp27 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser82 of human Hsp27 was used as immunogen. The antibody only detects Hsp27 phosphorylated on Serine 82. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml protein A purified Rabbit ascites. |
| Uses: | WB: 1:5,000IHC: 1:250ICC: 1:100IP: 1:30 |
| Background: | Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | anti-CMT2F antibody, anti-DKFZp586P1322 antibody, anti-Estrogen regulated 24 kDa protein antibody, anti-Heat shock 25kDa protein 1 antibody, anti-Heat shock 27kDa protein 1 antibody, anti-Heat shock 28kDa protein 1 antibody, anti-Heat Shock Protein 27 antibody, anti-Heat shock protein beta 1 antibody, anti-Heat Shock Protein27 antibody, anti-HS.76067 antibody, anti-Hsp 25 antibody, anti-Hsp 27 antibody, anti-Hsp 28 antibody, anti-Hsp B1 antibody, anti-Hsp25 antibody, anti-Hsp28 antibody, anti-HspB1 antibody, anti-SRP27 antibody, anti-Stress responsive protein 27 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Mendelsohn, M.E., et al. The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein. Proc Natl Acad Sci U S A. 88: 11212-6 (1991). 2. Faucher, C., et al. The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein. J. Biol Chem. 268: 15168-73 (1993). 3. Landry, J., et al. Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II. J. Biol. Chem. 267: 794-803 (1992). 4. Rouse, J., et al. A novel kinase cascade triggered by stress and heat shock that stimulates MAPKAP kinase-2 and phosphorylation of the small heat shock proteins. Cell 78: 1027-1037 (1994). |
| Phosphorylated: | Ser82 |
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