| CatalogCode: | NB110-57061 |
| ProductName: | HSP27 Phospho (pS78) Antibody |
| Product Description: | Rabbit Monoclonal anti-HSP27 Phospho (pS78) (Y175) [Ser78] |
| Clone: | Y175 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27 was used as immunogen. The antibody will detect Src phosphorylation on Serine 78. |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2,000IHC: 1:250-500ICC: 1:250-500IP: 1:50 |
| Background: | Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | anti-CMT2F antibody, anti-DKFZp586P1322 antibody, anti-Estrogen regulated 24 kDa protein antibody, anti-Heat shock 25kDa protein 1 antibody, anti-Heat shock 27kDa protein 1 antibody, anti-Heat shock 28kDa protein 1 antibody, anti-Heat Shock Protein 27 antibody, anti-Heat shock protein beta 1 antibody, anti-Heat Shock Protein27 antibody, anti-HS.76067 antibody, anti-Hsp 25 antibody, anti-Hsp 27 antibody, anti-Hsp 28 antibody, anti-Hsp B1 antibody, anti-Hsp25 antibody, anti-Hsp28 antibody, anti-HspB1 antibody, anti-SRP27 antibody, anti-Stress responsive protein 27 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Mendelsohn, M.E., et al. Proc Natl Acad Sci U S A. 88: 11212-6 (1991). 2. Faucher, C., et al. J. Biol Chem. 268: 15168-73 (1993). 3. Landry, J., et al. J. Biol. Chem. 267: 794-803 (1992). 4. Rouse, J., et al. Cell 78: 1027-1037 (1994). |
| Phosphorylated: | Ser78 |
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