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| Catalog Code: | NB110-57057 |
| Product Name: | HSD17B1 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-HSD17B1 (C-term) (EP1682Y) |
| Clone: | EP1682Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues on the C-terminus of human HSD17B1 was used as an immunogen. |
| Cross Reactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:100,000IHC: 1: 100 - 250ICC: 1:100 - 250FC: 1:15IP: 1:100 |
| Background: | The principal human estrogen, 17 beta-estradiol, is a potent stimulator of certain endocrine-dependent forms of breast cancer. Because human estrogenic 17 beta-hydroxysteroid dehydrogenase (HSD17B1) catalyzes the last step in the biosynthesis of 17 beta-estradiol from the less potent estrogen, estrone, it is an attractive target for the design of inhibitors of estrogen production and tumor growth (1). It is concluded that the steroid-binding site of human placental HSD17B1 contains a histidine residue which proximates the upper A-ring region of the steroid as it undergoes the reversible binding step (2). Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies (3). Defects in the conversion of androstenedione to testosterone in the fetal testes by the enzyme HSD17B1 give rise to genetic males with female external genitalia (4) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-17 beta hydroxysteroid dehydrogenase type 1 antibody, anti-EDH17B2 antibody, anti-EDHB17 antibody, anti-Estradiol 17 beta dehydrogenase 1 antibody, anti-HSD17 antibody, anti-Hydroxysteroid (17 beta) dehydrogenase 1 antibody, anti-Placental 17 beta hydroxysteroid dehydrogenase antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Murdock GL, et al. J Biol Chem 258(19):11460-4, 1983. 2. Ghosh D, et al. Structure 3(5):503-13, 1995. 3. Huang YW, et al. Mol Endocrinol 15(11):2010-20, 2001 4. Geissler WM, et al. Nat Genet 7(1):34-9, 1994. |
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