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| Catalog Code: | NB110-57051 |
| Product Name: | Histone H3TB Acetyl K14 Antibody |
| Product Description: | Rabbit Monoclonal anti-Histone H3TB Acetyl K14 (EP964Y) |
| Clone: | EP964Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic acetyl-specific peptide corresponding to residues around Lysine 14 was used as an immunogen. This antibody recognizes Histone H3TB acetylated on K14. |
| Cross Reactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2000IHC: 1:100 - 250ICC: 1:250 - 500IP: 1:20 |
| Background: | Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-H4F antibody, anti-HIST1H4 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Braunstein M, et al. Mol. Cell. Biol. 16:4349-56, 1996. 2. Workman JL, et al. Annu. Rev. Biochem. 67: 545- 579, 1998. 3. Cheung, P. et al. Cell 103, 263-27, 2000. 4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005. 5. Albig W, et al. Hum Genet 97:486-491. 1996. |
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