| CatalogCode: | NB110-57050 |
| ProductName: | Histone H3T, Acetyl (K27) Antibody |
| Product Description: | Rabbit Monoclonal anti-Histone H3T, Acetyl (K27) (EP865Y) |
| Clone: | EP865Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic acetylated peptide corresponding to residues surrounding Lys 27 of Histone H3T was used as immunogen. The antibody only detects Histone H3T acetylated on Lysine 27. |
| CrossReactivity: | Reacts in human. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500IHC: 1:100 - 250ICC: 1:100 - 250 |
| Background: | Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). . H3t (H.34) differs from the consensus mammalian H3 structure by four amino acid residues and it is mainly expressed in testicular cells. H3t encoding gene maps to chromosome 1 (1q42), unlike the majority of the human histone genes that form cluster on chromosome 6 and outside of replication-dependent H3 genes (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu |
| ALTnames: | anti-H3.4 antibody, anti-H3/g antibody, anti-H3FT antibody, anti-H3t antibody, anti-HIST3H3 antibody, anti-Histone 3 antibody, anti-H3 antibody, anti-Histone cluster 3 antibody, anti-H3 antibody, anti-MGC126886 antibody, anti-MGC126888 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Braunstein M, et al. Mol. Cell. Biol. 16:4349-56, 1996. 2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545-579, 1998. 3. Cheung, P. et al. Cell103, 263-27, 2000. 4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005. 5. Albig W, et al. Hum Genet 97:486-491. 1996. |
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