| request information: |
|
| Catalog Code: | NB110-57048 |
| Product Name: | Histone H3 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-Histone H3 (pS10) (E173) [Ser10] |
| Clone: | E173 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic phospho peptide corresponding to residues surrounding Serine 10 of Human Histone H3. |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species, based on sequence homology. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody works for IHC, IP, WB, ICC and is unsuitable for FACS. |
| Localization: | Nuclear |
| Background: | Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.05% BSA. |
| Application Summary: | immunohistochemistry 1:50-1:100, immunoprecipitation 1:40, Western Blot 1:2, Immunocytochemistry 1:250 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-H3 3 like sequence MH921 antibody; anti-H3 3A antibody; anti-H3 a antibody; anti-H3 b antibody; anti-H3 c antibody; anti-H3 d antibody; anti-H3 f antibody; anti-H3 h antibody; anti-H3 histone family member E pseudogene antibody; anti-H3 i antibody; anti-H3 j antibody; anti-H3 k antibody; anti-H3 l antibody; anti-H3F3 antibody; anti-Histone H3 3 pseudogene antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser10 |
| General References: | 1. Braunstein, M., et al. Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern. Mol. Cell. Biol. 16: 4349-56 (1996). 2. Workman, J.L. and R.E. Kingston. Alteration of nucleosome structure as a mechanism of transcriptional regulation. Annu. Rev. Biochem. 67: 545-579 (1998). 3. Hansen, J.C. et al. Structure and function of the core histone N-termini: more than meets the eye. Biochemistry 37, 17637-17641 (1998). 4. Strahl, B.D. and C.D. Allis. The language of covalent histone modifications. Nature 403, 41-45 (2000). |
order or
more information: | company product webpage |