| CatalogCode: | NB110-57040 |
| ProductName: | Histone H2B, Acetyl (K5) Antibody |
| Product Description: | Rabbit Monoclonal anti-Histone H2B, Acetyl (K5) (EP857Y) |
| Clone: | EP857Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 5. |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:50,000IHC: 1:250 - 500ICC: 1:250 - 500IP: 1:50 |
| Background: | Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Braunstein M, et al. Mol. Cell. Biol. 16:4349-56, 1996. 2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545-579, 1998. 3. Cheung, P. et al. Cell103, 263-27, 2000. 4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005. 5. Ahn SH, et al. Cell Cycle 4:780-783, 2005. |
order or
more information: | company product webpage |
| request information: |
|
|