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| Catalog Code: | NB110-57037 |
| Product Name: | Histone H2B Antibody |
| Product Description: | Rabbit Monoclonal anti-Histone H2B (EP819Y) |
| Clone: | EP819Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues of human Histone H2B was used as an immunogen. |
| Cross Reactivity: | Reacts in human and mouse. Does not react in rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB; 1:10,000IHC 1:100 - 250IP: 1:50 |
| Background: | Changes in chromatin structure play a key role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is acetylated in the N-terminal region, primary at lysines 5, 12, 15, and 20 (3). Ubiquitylation of histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of cell apoptosis and meiosis (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BS |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-H2B histone family member O antibody, H2B histone family member S antibody, H2B.1 antibody, H2B.1 B antibody, H2B.b antibody, H2B.c antibody, H2B.d antibody, H2B.e antibody, H2B.f antibody, H2B.j antibody, H2B.q antibody, H2B/b antibody, H2B/c antibody, H2B/d antibody, H2B/e antibody, H2B/f antibody, H2B/j antibody, H2B/o antibody, H2B/q antibody, H2BFB antibody, H2BFC antibody, H2BFD antibody, H2BFE antibody, H2BFF antibody, H2BFJ antibody, H2BFO antibody, H2BFQ antibody, H2BFS antibody, HIRIP2 antibody, HIST1H2BB antibody, HIST1H2BD antibody, HIST1H2BH antibody, HIST1H2BL antibody, HIST1H2BM antibody, HIST1H2BN antibody, HIST2H2BE antibody, Histone H2B antibody, Histone H2B type 1 B antibody, Histone H2B type 1 D antibody, Histone H2B type 1 H antibody, Histone H2B type 1 L antibody, Histone H2B type 1 M antibody, Histone H2B type 1 N antibody, Histone H2B type 2 E antibody, Histone protein antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.25% sodium azide |
| General References: | 1. Braunstein M, et al. Mol. Cell. Biol. 16:4349-56, 1996. 2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67: 545-579, 1998. 3. Cheung, P. et al. Cell103, 263-27, 2000. 4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005. 5. Ahn SH, et al. Cell Cycle 4:780-783, 2005. |
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