| CatalogCode: | NB110-57031 |
| ProductName: | HIF-1 alpha Antibody |
| Product Description: | Rabbit Monoclonal anti-HIF-1 alpha (EP1215Y) |
| Clone: | EP1215Y |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide corresponding to residues near the C terminal of human HIF1 alpha. |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human HIF-1 alpha was used as an immunogen. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody works for IHC, WB, and ICC. |
| Localization: | Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. |
| Control: | Ramos + CoCl2 lysate and liver carcinoma. |
| Background: | The HIF1A gene encodes the HIF-1alpha subunit of hypoxia-inducible factor 1, a transcription factor that is essential for cardiovascular development and systemic O2 homeostasis (1). HIF-1 is found in mammalian cells cultured under reduced O2 tension and is necessary for transcriptional activation mediated by the erythropoietin gene enhancer in hypoxic cells. It has been shown that both HIF-1 subunits are basic-helix-loop-helix proteins containing a PAS domain, defined by its presence in the in the mammalian ARNT and AHR proteins. HIF-1 alpha is most closely related to Sim (2). HIF-1 binds to cis-acting hypoxia-response elements within the erythropoietin, vascular endothelial growth factor, and other genes to activate transcription in hypoxic cells. Results indicate that increased transcriptional activity mediated by HIF-1 in hypoxic cells results from both increased HIF-1alpha protein levels and increased activity of HIF-1alpha transactivation domains (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | anti-HIF-1 alpha; anti-HIF1 alpha; anti-ARNT-interacting protein; anti-Member of PAS protein 1; anti-Basic-helix-loop-helix-PAS protein MOP1 |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Iyer NV, et al. Genomics 52(2):159-65, 1998. 2. Wang GL, et al. Proc Natl Acad Sci 92(12):5510-4, 1995. 3. Jiang BH, et al. J Biol Chem 272(31):19253-60, 1997. |
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